Hi all,
Here is an unconventional question for Photoshop experts.
Here are two pictures (click for larger image)
These are cell cultures taken from under a microscope. They are particularly bad images as we are waiting for a better suited camera (note all the distortion, flaring and uneven lighting)
I have discovered that I can use Photoshop to determine the percentage of the image covered by cells (this is called cell confluence) by selecting the cell culture using magic wand and using the histogram to determine the amount of pixels selected. The confluence can then be derived.
I have been using a few techniques like contrast, equalizer and lens correction to retouch the pictures, but this still yields bad results.
Would you have any suggestions to further improve this technique.
Thanks
Here is an unconventional question for Photoshop experts.
Here are two pictures (click for larger image)
These are cell cultures taken from under a microscope. They are particularly bad images as we are waiting for a better suited camera (note all the distortion, flaring and uneven lighting)
I have discovered that I can use Photoshop to determine the percentage of the image covered by cells (this is called cell confluence) by selecting the cell culture using magic wand and using the histogram to determine the amount of pixels selected. The confluence can then be derived.
I have been using a few techniques like contrast, equalizer and lens correction to retouch the pictures, but this still yields bad results.
Would you have any suggestions to further improve this technique.
Thanks