Hi
@Remousamavi
Yes very likely that there was a colorspace conversion (that includes grayscale conversions) where the tone response curve (also known as gamma) was different.
The key is either to avoid the conversion or making sure the conversion is done between spaces that have the same tone response curve.
To be more specific, it would be helpful to know
- Are you using Photoshop CC or some other program in which you are editing
- Are you editing in Grayscale mode or in RGB mode where R=G=B for each pixel
- In which color space are you editing e.g. If RGB Adobe RGB, sRGB etc or if in Grayscale what Gamma or Dot Gain space are you using
- What specific command are you using in Photoshop e.g Save As, Export, Save for Web and maybe a screen shot of the settings and/or preferences if using the Export command
- Are you in Photoshops 16 bit mode (that would be my assumption yet double checking)
- Are you saving/exporting also to RGB mode or converting to Grayscale mode and which RGB/Gray space are you converting too.
- After saving the file, which application will be using the file. The receiving application may required a given color/gray space and may be doing a conversion on input.
Without the details of above, if you are staying in RGB or Grayscale, the easiest way to avoid a conversion is to uncheck the box that says convert to sRGB
As additional information or tidbits
Photoshop in 16 bit mode actually only supports 15 bits (it rounds the lower bit) - not sure if that is important to you or not
Save for web I believe converts to 8 bit mode (at least it used to)
Not all export formats support 16 bits. PNG and PBM does yet I am pretty sure BMP does not. Is preserving 16 bit data important to you?
I noticed you are using hex format in 16 bit mode. I have not seen 16bit grayscale representation in hex in the info panel or in the color checker. I may be missing something on how to do that in Photoshop if you would like to share how you do that.